TRYPSIN-ORCEIN BANDING IN PLANT CHROMOSOMES

被引：4

作者：

LAVANIA, UC

SHARMA, AK

机构：

[1] Cytogenetics Laboratory, Department of Botany, University of Calcutta, Calcutta

来源：

STAIN TECHNOLOGY | 1979年 / 54卷 / 05期

关键词：

D O I：

10.3109/10520297909110682

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A convenient and quick method using trypsin-orcein for handing plant chromosomes (O-banding) is suggested. The technique is directly applicable to meristematic tissues (e.g. root tip) and involves the treatment of root tip with 1-2% solution of trypsin either in buffer or in 05 N HCI for 5-10 minutes at 37 C or for 30-60 minuta near 0 C followed by staining with 1.5% acetic orcein: 1 N HCI (19:1). Dark staining bands are reproducible and species specific. These bands possibly represent specific DNA-protein-dye interaction. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：261 / 263

页数：3

共 8 条

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SHARMA AK, 1977, FRONTIERS PLANT SCI, P181

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