5-S RIBOSOMAL-RNA IS CONTAINED WITHIN A 25-S RIBOSOMAL-RNA THAT ACCUMULATES IN MUTANTS OF ESCHERICHIA-COLI DEFECTIVE IN PROCESSING OF RIBOSOMAL-RNA

A temperature-sensitive mutant strain of Escherichia coli defective in two RNA processing enzymes, RNase III and RNase E (rnc. rne), fails to produce normal levels of 23 S and 5 S rRNA at the non-permissive temperature. Instead, a molecule larger than 23 S is produced. This molecule, designated 25 S rRNA, can be processed in vitro to produce p5 rRNA. These findings further our understanding of the overall processing events of ribosomal RNA which take place in the bacterial cell. © 1979.