Sandwich Enzyme-Linked Immunosorbent Assays (SELISAs) Quantitate and Distinguish Two Forms of Transforming Growth Factor-Beta (TGF-beta 1 and TGF-beta 2) in Complex Biological Fluids

被引：127

作者：

Danielpour, David ^{[1
]}

Kim, Kyung-Young ^{[1
]}

Dart, Linda L. ^{[1
]}

Watanabe, Shinichi ^{[1
]}

Roberts, Anita B. ^{[1
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NCI, Lab Chemoprevent, Bldg 41,Rm C629, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1989年 / 2卷 / 01期

关键词：

transforming growth factor-beta; sandwich ELISA; turkey antibodies; rabbit antibodies; affinity purification; conditioned medium; trichloroacetic acid precipitation;

D O I：

10.3109/08977198909069082

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed sandwich enzyme-linked immunosorbent assays (SELISAs) for TGF-beta 1 and TGF-beta 2 using both turkey and rabbit neutralizing polyclonal antibodies against native TGF-beta s. Each assay is based on the binding of two different antibodies to distinct epitopes of a single TGF-beta molecule. With these assays, TGF-beta types 1 and 2 can each be specifically quantitated in complex biological fluids, with detection limits of 2-5 pg. TGF-beta 3 and TGF-beta 5 either do not cross-react or cross-react very poorly in these assays. TGF-$1.2 heterodimer, although 50-80% neutralized by either TGF-beta 1 or TGF-beta 2 antibodies, shows only a 1.5 and 3.7% cross-reactivity in the TGF-beta 1 and TGF-beta 2 SELISAs, respectively. The SELISAs reported here represent the most specific, rapid, and precise assays for TGF-beta 1 and TGF-beta 2 reported thus far.

引用

页码：61 / 71

页数：11

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