PREPARATIVE COUNTERCURRENT CHROMATOGRAPHY WITH A SLOWLY ROTATING HELICAL TUBE

被引：16

作者：

ITO, Y ^{[1
]}

BOWMAN, RL ^{[1
]}

机构：

[1] NATL HEART LUNG & BLOOD INST, TECH DEV LAB, BETHESDA, MD 20014 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1977年 / 136卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(00)86271-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The capability of a simple countercurrent chromatographic scheme for obtaining high-resolution preparative-scale separations was demonstrated with the separations of a series of dinitrophenyl (DNP) amino acids and peptides. Basic studies on stationary phase retention and partition efficiency with a low-viscosity chloroform-acetic acid-0.1 N HCl (2:2:1) phase system together with the results previously obtained with a viscous n-butanol system suggest a general applicability of low-interfacial tension phase systems by the present method.

引用

页码：189 / 198

页数：10

共 5 条

[1] COUNTERCURRENT CHROMATOGRAPHY [J].

ITO, Y ;

HURST, RE ;

BOWMAN, RL ;

ACHTER, EK .

SEPARATION AND PURIFICATION METHODS, 1974, 3 (01) :133-165

[2] COUNTERCURRENT CHROMATOGRAPHY [J].

ITO, Y ;

BOWMAN, RL .

ANALYTICAL CHEMISTRY, 1971, 43 (13) :A69-&

[3]

Ito Y., IN PRESS

[4] THE CHROMATOGRAPHIC FRACTIONATION OF RIBONUCLEASE [J].

MARTIN, AJP ;

PORTER, RR .

BIOCHEMICAL JOURNAL, 1951, 49 (02) :215-218

[5] PARTITION CHROMATOGRAPHY OF INSULIN AND OTHER PROTEINS [J].

PORTER, RR .

BIOCHEMICAL JOURNAL, 1953, 53 (02) :320-328

← 1 →