ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF HIGHLY VIRULENT-STRAINS OF AEROMONAS-HYDROPHILIA AND AEROMONAS-SOBRIA IN WATER

被引：1
|
作者：
MERINO, S [1 ]
CAMPRUBI, S [1 ]
REGUE, M [1 ]
TOMAS, JM [1 ]
机构：
[1] UNIV BARCELONA,DEPT MICROBIOL,E-08071 BARCELONA,SPAIN
来源：
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1993年 / 8卷 / 04期
关键词：
D O I：
10.1002/tox.2530080409
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of Aeromonas hydrophila and sobria serotype 0:11 (highly virulent strains). The assay utilizes a detector antibody that shows no cross-reactions with Aeromonas strains not belonging to serotype 0:II or non-Aeromonas competing organisms. All the A. hydrophila and sobria strains from serotype 0 : II tested reacted strongly with the detector antibody. Also, by culturing and performing the immunoassay on a filter with the detector antibody, we can quantify A. hydrophilia or sobria 0:11 cells/100 mL of water. (c) 1993 John Wiley and Sons, Inc.
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页码：451 / 460
页数：10
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