ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF HIGHLY VIRULENT-STRAINS OF AEROMONAS-HYDROPHILIA AND AEROMONAS-SOBRIA IN WATER

被引：1
|
作者：
MERINO, S [1 ]
CAMPRUBI, S [1 ]
REGUE, M [1 ]
TOMAS, JM [1 ]
机构：
[1] UNIV BARCELONA,DEPT MICROBIOL,E-08071 BARCELONA,SPAIN
来源：
ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1993年 / 8卷 / 04期
关键词：
D O I：
10.1002/tox.2530080409
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of Aeromonas hydrophila and sobria serotype 0:11 (highly virulent strains). The assay utilizes a detector antibody that shows no cross-reactions with Aeromonas strains not belonging to serotype 0:II or non-Aeromonas competing organisms. All the A. hydrophila and sobria strains from serotype 0 : II tested reacted strongly with the detector antibody. Also, by culturing and performing the immunoassay on a filter with the detector antibody, we can quantify A. hydrophilia or sobria 0:11 cells/100 mL of water. (c) 1993 John Wiley and Sons, Inc.
引用
收藏
页码：451 / 460
页数：10
相关论文
共 50 条
  • [1] DETECTION OF AEROMONAS-HYDROPHILA IN FOOD WITH AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY
    MERINO, S
    CAMPRUBI, S
    TOMAS, JM
    JOURNAL OF APPLIED BACTERIOLOGY, 1993, 74 (02): : 149 - 154
  • [2] ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR AEROMONAS-HYDROPHILA HEMOLYSINS
    KOZAKI, S
    KUROKAWA, A
    ASAO, T
    KATO, K
    UEMURA, T
    SAKAGUCHI, G
    FEMS MICROBIOLOGY LETTERS, 1987, 41 (02) : 147 - 151
  • [3] DETECTION OF ANTIBODY AGAINST AEROMONAS-SALMONICIDA IN THE SERUM OF SALMONID FISH BY THE ENZYME-LINKED-IMMUNOSORBENT-ASSAY
    YOSHIMIZU, M
    DIREKBUSARAKOM, S
    NOMURA, T
    EZURA, Y
    KIMURA, T
    FISH PATHOLOGY, 1992, 27 (02): : 73 - 82
  • [4] DETECTION OF AEROMONAS-HYDROPHILA SEROGROUP 0/34 IN FECES USING AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY
    MERINO, S
    CAMPRUBI, S
    TOMAS, JM
    JOURNAL OF DIARRHOEAL DISEASES RESEARCH, 1993, 11 (01): : 30 - 34
  • [5] Enzyme-linked immunosorbent assay for detection of Aeromonas hydrophila serogroup O:19
    Sendra, RM
    Esteve, C
    Alcaide, E
    FEMS MICROBIOLOGY LETTERS, 1997, 157 (01) : 123 - 129
  • [6] DETECTION OF MOLD IN FOOD BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY
    NOTERMANS, S
    HEUVELMAN, CJ
    VANEGMOND, HP
    PAULSCH, WE
    BESLING, JR
    JOURNAL OF FOOD PROTECTION, 1986, 49 (10) : 786 - 791
  • [7] DIRECT DETECTION OF RHINOVIRUSES BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY
    DEARDEN, CJ
    ALNAKIB, W
    JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (02) : 179 - 189
  • [8] AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR RESIDUE DETECTION OF METHOPRENE
    MEI, JV
    YIN, CM
    CARPINO, LA
    ACS SYMPOSIUM SERIES, 1990, 442 : 140 - 155
  • [9] DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR ISOPROTURON IN WATER
    KATMEH, MF
    FROST, G
    AHERNE, W
    STEVENSON, D
    ANALYST, 1994, 119 (03) : 431 - 435
  • [10] AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF MARINE CALICIVIRUSES
    FERRIS, NP
    OXTOBY, JM
    VETERINARY MICROBIOLOGY, 1994, 42 (2-3) : 229 - 238
12345