GLIADIN ALLELES IN CANADIAN WESTERN RED SPRING WHEAT CULTIVARS - USE OF 2 DIFFERENT PROCEDURES OF ACID POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR GLIADIN SEPARATION

被引:13
作者
METAKOVSKY, EV
NG, PKW
CHERNAKOV, VM
BUSHUK, W
机构
[1] MICHIGAN STATE UNIV, DEPT FOOD SCI & HUMAN NUTR, E LANSING, MI 48824 USA
[2] NI VAVILOW INST GEN GENET, MOSCOW B-333, RUSSIA
[3] UNIV MANITOBA, DEPT FOOD SCI, WINNIPEG R3T 2N2, MANITOBA, CANADA
关键词
GLIADIN ALLELES; ACID POLYACRYLAMIDE GEL ELECTROPHORESIS; CANADIAN BREAD WHEATS;
D O I
10.1139/g93-099
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Gliadin allele compositions of 21 Canadian spring common wheat cultivars, most of which belong to the Canada western red spring (CWRS) class, were studied and great similarity in their genotypes was confirmed. It was found that alleles frequent in the set of Canadian wheats (such as Gli-B1d, Gli-D1j, Gli-A2m, and Gli-D2h) are very rare or absent in common wheat cultivars from other regions and countries studied earlier, indicating that germplasm of CWRS cultivars is rather unique. It may be suggested that alleles frequent in Canadian cultivars relate to important technological characteristics of these wheats and may possibly serve as marker genes during selection for quality traits. Similarity of gliadin electrophoregrams obtained by two different acid polyacrylamide gel electrophoretic procedures for the same genotype was established, and the component composition of allelic variants of blocks of gliadin components found in the set of Canadian cultivars and in standard cultivars Chinese Spring and Bezostaya 1 are described.
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页码:743 / 749
页数:7
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