POLYMER PROTEIN CONJUGATES .1. EFFECT OF PROTEIN CONJUGATION ON THE CLOUD POINT OF POLY(N-ISOPROPYLACRYLAMIDE)

被引：85
|
作者：
CHEN, JP [1 ]
YANG, HJ [1 ]
HOFFMAN, AS [1 ]
机构：
[1] UNIV WASHINGTON,CTR BIOENGN,FL-20,SEATTLE,WA 98195
来源：
BIOMATERIALS | 1990年 / 11卷 / 09期
关键词：
polymer-protein conjugates; polymers; Proteins;
D O I：
10.1016/0142-9612(90)90019-M
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Poly(N-isopropylacrylamide) (p(NIPAAm)) exhibits a cloud point around 32°C. In this study we have conjugated several proteins to p(NIPAAm), and the effects of protein molecular weight, pH and ionic strength on the cloud point of the p(NIPAAm)-protein conjugates have been studied. The cloud points of p(NIPAAm)-protein conjugates appear to be unaffected by the molecular weight or isoelectric point (pi) of the proteins, the pH or ionic strength. © 1990.
引用
收藏
页码：625 / 630
页数：6
相关论文
共 50 条
  • [1] EFFECT OF POLYMERIC SURFACTANTS ON THE CLOUD POINT OF POLY(N-ISOPROPYLACRYLAMIDE)
    CHO, CS
    JUNG, JH
    SUNG, YK
    LEE, YM
    MACROMOLECULAR RAPID COMMUNICATIONS, 1994, 15 (09) : 727 - 732
  • [2] Cloud point of poly(N-isopropylacrylamide) synthesized in the presence of cyclodextrin
    Han, SJ
    Yoo, MK
    Sung, YK
    Lee, YM
    Cho, CS
    MACROMOLECULAR RAPID COMMUNICATIONS, 1998, 19 (08) : 403 - 407
  • [3] Zein-poly(N-isopropylacrylamide) conjugates
    Bromberg, L
    JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (04): : 504 - 507
  • [4] Fluorescent semiconducting polymer conjugates of poly(N-isopropylacrylamide) for thermal precipitation assays
    Kuroda, K
    Swager, TM
    MACROMOLECULES, 2004, 37 (03) : 716 - 724
  • [5] POLYMER PROTEIN CONJUGATES .2. AFFINITY PRECIPITATION SEPARATION OF HUMAN IMMUNO-GAMMA-GLOBULIN BY A POLY(N-ISOPROPYLACRYLAMIDE)-PROTEIN-A CONJUGATE
    CHEN, JP
    HOFFMAN, AS
    BIOMATERIALS, 1990, 11 (09) : 631 - 634
  • [6] Highly branched poly(N-isopropylacrylamide) for use in protein purification
    Carter, S
    Rimmer, S
    Rutkaite, R
    Swanson, L
    Fairclough, JPA
    Sturdy, A
    Webb, M
    BIOMACROMOLECULES, 2006, 7 (04) : 1124 - 1130
  • [7] Is a "Cloud-Point Curve" in Aqueous Poly(N-isopropylacrylamide) Solution Binodal?
    Kawaguchi, Tomoaki
    Kobayashi, Kunihiko
    Osa, Masashi
    Yoshizaki, Takenao
    JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (16): : 5440 - 5447
  • [8] EFFECT OF CONJUGATION OF PHOSPHOLIPID TO POLY(N-ISOPROPYLACRYLAMIDE) ON ITS CRITICAL SOLUTION TEMPERATURE
    WU, XS
    HOFFMAN, AS
    YAGER, P
    MAKROMOLEKULARE CHEMIE-RAPID COMMUNICATIONS, 1993, 14 (05): : 309 - 314
  • [9] Poly(N-isopropylacrylamide) branched polymer nanoparticles
    Chambon, Pierre
    Chen, Lin
    Furzeland, Steve
    Atkins, Derek
    Weaver, Jonathan V. M.
    Adams, Dave J.
    POLYMER CHEMISTRY, 2011, 2 (04) : 941 - 949
  • [10] N-ISOPROPYLACRYLAMIDE (NIPAAM) AND N-ACRYLOXYSUCCINIMIDE (NASI) COPOLYMER - A WATER-SOLUBLE, ACTIVATED POLYMER FOR PROTEIN CONJUGATION
    COLE, CA
    SCHREINER, SM
    MONJI, N
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1986, 191 : 205 - POLY
12345