NONTOXIGENIC STRAINS OF CLOSTRIDIUM-DIFFICILE LACK THE GENES FOR BOTH TOXIN-A AND TOXIN-B

被引：41

作者：

FLUIT, AC ^{[1
]}

WOLFHAGEN, MJHM ^{[1
]}

VERDONK, GPHT ^{[1
]}

JANSZE, M ^{[1
]}

TORENSMA, R ^{[1
]}

VERHOEF, J ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,U-GENE RES BV,3584 CS UTRECHT,NETHERLANDS

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 11期

关键词：

D O I：

10.1128/JCM.29.11.2666-2667.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 39 toxigenic and 20 nontoxigenic strains of Clostridium difficile were tested for the presence of either toxin A or toxin B by the polymerase chain reaction (PCR). All toxigenic strains produced cytotoxin as assayed by using highly sensitive fetal lung fibroblasts and were positive for toxin A as well as toxin B in the PCR assay. All nontoxigenic strains failed to produce toxin and were negative in the PCR assay. This study shows that nontoxigenic strains of Clostridium difficile lack the toxin A as well as the toxin B gene.

引用

页码：2666 / 2667

页数：2

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