N-TERMINAL CYSTEINES ESSENTIAL FOR GOLGI SORTING OF B-50 (GAP-43) IN PC12 CELLS

被引：12
|
作者：
AARTS, LHJ
VANDERLINDEN, JAM
HAGE, WJ
VANROZEN, AJ
OESTREICHER, AB
GISPEN, WH
SCHOTMAN, P
机构：
[1] UNIV UTRECHT,RUDOLF MAGNUS INST NEUROSCI,DEPT MED PHARMACOL,3584 CG UTRECHT,NETHERLANDS
[2] UNIV UTRECHT,NETHERLANDS INST DEV BIOL,3584 CG UTRECHT,NETHERLANDS
来源：
NEUROREPORT | 1995年 / 6卷 / 07期
关键词：
B-50; GAP-43; NEUROMODULIN; GOLGI APPARATUS; PROTEIN SORTING; PC12; CELLS; WHEAT GERM AGGLUTININ; CONFOCAL MICROSCOPY; MUTATION;
D O I：
10.1097/00001756-199505090-00005
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
WE have examined the subcellular distribution of the growth-associated protein B-50 (GAP-43) in pheochromocytoma (PC12) cells, using confocal microscopy. Proliferating PC12 cells contained very low levels of B-50 in the cytosol. Enhanced expression of B-50 in these cells, evoked by either nerve growth factor (NGF) treatment or transient transfection with rat B-50 cDNA, led to Golgi sorting and membrane targeting of the B-50 protein. Site directed mutagenesis of Cys(3)Cys(4) to Ser,Gly, in B-50 resulted in a cytosolic distribution. We conclude that Cys(3) and Cys(4) are essential for accumulation of B-50 both at the plasma membrane and in the Golgi apparatus of PC12 cells.
引用
收藏
页码：969 / 972
页数：4
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