2 GENES INVOLVED IN THE PHASE-VARIABLE PHI-C31 RESISTANCE MECHANISM OF STREPTOMYCES-COELICOLOR A3(2)

The phage growth limitation (Pgl) system of Streptomyces coelicolor confers resistance to phi C31 and its homoimmune phages, The positions of the pgl genes within a 16 kb clone of S. coelicolor DNA were defined by subcloning, insertional inactivation, and deletion mapping, Nucleotide sequencing and functional analysis identified two genes, pglY and pglZ, required for the Pgl(+) (phage-resistant) phenotype, pglY and pglZ, which mag. be translationally coupled, are predicted to encode proteins with M(r)s of 141,000 and 101,000, respectively, Neither protein shows significant similarity to other known proteins, but PglY has a putative ATP/GTP binding motif. The pglY and pglZ genes are cotranscribed from a single promoter which appears to be constitutive and is not induced by phage infection.