Cyathisterone (ergosta-7,22-diene-3,6-dione) and cyathisterol (8 beta-hydroxyergosta-4,6,22-trien-3-one), two new steroids, have been isolated from Calvatia cyathiformis along with two known ergosterol derivatives, ergosta-4,7,22-triene-3,6-dione and ergosta-4,6,8(14),22-tetraen-3-one. Their molecular structures were defined by spectroscopic means and chemical correlations.