A SIMPLE METHOD FOR THE DIRECT EXTRACTION OF PLASMID DNA FROM YEAST

被引：6

作者：

SOBANSKI, MA

DICKINSON, JR

机构：

[1] School of Pure and Applied Biology, University of Wales College of Cardiff, Cardiff, CF1 3TL

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 03期

关键词：

D O I：

10.1007/BF00157083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple method for the small scale isolation of shuttle plasmid DNA from Saccharomyces cerevisiae is described. It uses glass beads to break cells and reagents which are also used in bacterial mini-preps to yield plasmid DNA without chromosomal contamination in sufficient quantities to enable direct visualisation on agarose gels.

引用

页码：225 / 230

页数：6

共 12 条

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