MEASUREMENT OF INTRACELLULAR FREE CA2+ CONCENTRATION IN GUINEA-PIG SPIRAL GANGLION-CELLS

被引：10

作者：

SHIMOZONO, M ^{[1
]}

TONO, T ^{[1
]}

MORIMITSU, T ^{[1
]}

NAKAGAWA, T ^{[1
]}

KOMUNE, S ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT OTORHINOLARYNGOL,FUKUOKA 812,JAPAN

来源：

NEUROREPORT | 1995年 / 6卷 / 03期

关键词：

INTRACELLULAR FREE CA2+; FLUORESCENT STUDY; GUINEA PIG; FURA-2; SPIRAL GANGLION CELL; CA2+ CHANNEL; EXCITATORY AMINO ACID;

D O I：

10.1097/00001756-199502000-00004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

INTRACELLULAR free Ca2+ concentration ([Ca2+](i)) in spiral ganglion cells (SGC) of guinea pig was measured using the fura-2 fluorescence method. Application of high-K+ external solution increased [Ca2+](i). [Ca2+](i) elevation was suppressed by either depletion of external Ca2+ or application of the dihydropyridine Ca2+ channel antagonist, nicardipine. One of the putative hair cell afferent nerve neurotransmitters, L-glutamate (Glu), induced [Ca2+](i) elevation in SGC. Pharmacological studies suggested that the Glu receptor may be a non-NMDA subtype. Glu-induced response was suppressed by either depletion of external Ca2+ or application of nicardipine, indicating that Glu-induced [Ca2+](i) elevation is mainly carried out by the voltage-dependent Ca2+ channel in SGC.

引用

页码：421 / 424

页数：4

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