THE NEGLIGIBLE ROLE OF CARBON-DIOXIDE AND ETHYLENE IN AJMALICINE PRODUCTION BY CATHARANTHUS-ROSEUS CELL-SUSPENSIONS

被引：22
|
作者：
SCHLATMANN, JE
FONCK, E
TENHOOPEN, HJG
HEIJNEN, JJ
机构：
[1] Biotechnology Delft Leiden, Project Group Plant Cell Biotechnology Department of Biochemical Engineering, Delft University of Technology, BC Delft, 2628
来源：
PLANT CELL REPORTS | 1994年 / 14卷 / 2-3期
关键词：
AJMALICINE; CARBON DIOXIDE; ETHYLENE; BIOREACTOR; CATHARANTHUS ROSEUS;
D O I：
10.1007/BF00233781
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Removal of gaseous metabolites in an aerated fermenter affects ajmalicine production by Catharanthus roseus negatively. Therefore, the role of CO2 and ethylene in ajmalicine production by C. roseus was investigated in 31 fermenters (working volume 1.81) with recirculation of a large part of the exhaust air. Removal of CO2, ethylene or both from the recirculation stream did not have an effect on ajmalicine production. Inhibition of ethylene biosynthesis in shake flasks with Co2+, Ni2+ or aminooxyacetic acid did not affect ajmalicine production. However, the removal of CO2 did enhance the amount of extracellular ajmalicine.
引用
收藏
页码：157 / 160
页数：4
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