EFFECTS OF PRENATAL EXPOSURE TO COCAINE ON HEART-RATE AND NONASSOCIATIVE LEARNING AND RETENTION IN INFANT RATS

Nonassociative learning and retention were assessed in terms of habituation of a heart-rate (HR) orienting response (bradycardia) to a tone in 16-day-old male and female rats prenatally exposed to cocaine and in control offspring. Offspring were derived from Sprague-Dawley dams given daily subcutaneous injections of 40 mg/kg/3 cc cocaine HCl (C40) from gestational days 8-20, pair-fed control dams given saline injections (PF), and nontreated control dams (LC). Each pup was adapted to the test apparatus for 15 min prior to being given 10 presentations of a pulsing tone, each separated by a 65-sec intertrial interval, with HR measured during a 5-sec pretone period and throughout the 10-sec tone for each trial. To assess retention, subjects were given 10 additional tone trials either 1, 2, 4, or 6 hr later. C40 male offspring displayed significantly lower basal HR following the initial 15-min isolation period than either LC or PF offspring, whereas prenatal treatment had no effect on basal HR among females. Although no differences were seen in rate of habituation, prenatal cocaine exposure was observed to affect retention of the habituated orienting response. Whereas LC and PF offspring retained habituation of the orienting response for less than 4 hr, C40 offspring exhibited no forgetting after a 4-hr interval, and showed significant loss of the habituation response only after a 6-hr interval. (C) 1994 Wiley-Liss, Inc.