ESTIMATION OF AMOUNTS OF PHYTOPHTHORA-INFESTANS MYCELIUM IN LEAF TISSUE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：52
作者：
HARRISON, JG
BARKER, H
LOWE, R
REES, EA
机构：
[1] Scottish Crop Research Institute, Dundee, DD2 5DA, Invergowrie
来源：
PLANT PATHOLOGY | 1990年 / 39卷 / 02期
关键词：
D O I：
10.1111/j.1365-3059.1990.tb02503.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A polyclonal antiserum, prepared in a rabbit immunized with a mycelium extract of Phytophthora infestans, reacted in an enzyme‐linked immunosorbent assay (ELISA) with mycelial extracts of two Phytophthora species but not with those of 10 other micro‐organisms found on potato. P. infestans mycelium in leaf tissue was readily detected by ELISA using either the plate‐trapped antigen or F(ab')2 antibody‐fragment techniques. The amount of mycelium in leaf extracts was estimated by comparing the values obtained in ELISA with those for known concentrations of P. infestans mycelium. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：274 / 277
页数：4
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