FLOW CYTOMETRY OF HUMAN-SPERMATOZOA

被引：52
作者：
OTTO, FJ
HACKER, U
ZANTE, J
SCHUMANN, J
GOHDE, W
MEISTRICH, ML
机构：
[1] UNIV MUNSTER,FACHKLIN HORNHEIDE,EXPTL TUMORFORSCH ABT,D-4400 MUNSTER,FED REP GER
[2] UNIV TEXAS,MD ANDERSON HOSP & TUMOR INST,CTR CANC,EXPTL RADIOTHERAPY SECT,HOUSTON,TX 77030
来源：
HISTOCHEMISTRY | 1979年 / 61卷 / 03期
关键词：
D O I：
10.1007/BF00508445
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes and effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochromes is achieved. Thus reliable and precise measurements of the relative DNA content of human spermatozoa are possible and the two subpopulations of haploid spermatozoa can be distinguished according to the difference in their DNA content. © 1979 Springer-Verlag.
引用
收藏
页码：249 / 254
页数：6
相关论文
共 14 条
[1]  
BARLOGIE B, 1976, PULSE CYTOPHOTOMETRY, V2, P125
[2]  
CRISSMAN HA, 1976, PULSE CYTOPHOTOMETRY, V2, P112
[3]   HYDRODYNAMIC ORIENTATION OF SPERM HEADS FOR FLOW CYTOMETRY [J].
DEAN, PN ;
PINKEL, D ;
MENDELSOHN, ML .
BIOPHYSICAL JOURNAL, 1978, 23 (01) :7-13
[4]   DECONDENSATION OF SPERM NUCLEI INVITRO [J].
GALL, WE ;
OHSUMI, Y .
EXPERIMENTAL CELL RESEARCH, 1976, 102 (02) :349-358
[5]   FLOW MICROFLUOROMETRIC ANALYSIS OF SPERM DNA CONTENT - EFFECT OF CELL-SHAPE ON FLUORESCENCE DISTRIBUTION [J].
GLEDHILL, BL ;
LAKE, S ;
STEINMETZ, LL ;
GRAY, JW ;
CRAWFORD, JR ;
DEAN, PN ;
VANDILLA, MA .
JOURNAL OF CELLULAR PHYSIOLOGY, 1976, 87 (03) :367-375
[6]   RESOLUTION OF X-SPERMATIDS AND Y-SPERMATIDS BY PULSE CYTOPHOTOMETRY [J].
MEISTRICH, ML ;
GOHDE, W ;
WHITE, RA ;
SCHUMANN, J .
NATURE, 1978, 274 (5673) :821-823
[7]   DNA CONTENT AND DNA BASED CENTROMERIC INDEX OF 24 HUMAN CHROMOSOMES [J].
MENDELSOHN, ML ;
MAYALL, BH ;
BOGART, E ;
MOORE, DH ;
PERRY, BH .
SCIENCE, 1973, 179 (4078) :1126-1129
[8]  
MILETTE CF, 1973, J CELL BIOL, V58, P662
[9]   MODIFICATION OF DAPI BANDING ON HUMAN-CHROMOSOMES BY PRE-STAINING WITH A DNA-BINDING OLIGOPEPTIDE ANTIBIOTIC, DISTAMYCIN-A [J].
SCHWEIZER, D ;
AMBROS, P ;
ANDRLE, M .
EXPERIMENTAL CELL RESEARCH, 1978, 111 (02) :327-332
[10]   REVERSE FLUORESCENT CHROMOSOME-BANDING WITH CHROMOMYCIN AND DAPI [J].
SCHWEIZER, D .
CHROMOSOMA, 1976, 58 (04) :307-324
12