IMMOBILIZATION OF UREASE BY THIOL-DISULFIDE INTERCHANGE WITH CONCOMITANT PURIFICATION

被引：42
作者：
CARLSSON, J
AXEN, R
BROCKLEHURST, K
CROOK, EM
机构：
[1] UNIV LONDON, ST BARTHOLOMEWS HOSP,MED COLL,DEPT BIOCHEM & CHEM, CHARTERHOUSE SQ, LONDON EC1M 6BQ, ENGLAND
[2] UNIV UPPSALA, INST BIOCHEM, BOX 531, S-75121 UPPSALA, SWEDEN
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1974年 / 44卷 / 01期
关键词：
D O I：
10.1111/j.1432-1033.1974.tb03472.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：189 / 194
页数：6
相关论文
共 26 条
[1]  
ANDREWS A T D B, 1970, Archives of Biochemistry and Biophysics, V141, P538, DOI 10.1016/0003-9861(70)90172-4
[2]   CHEMICAL FIXATION OF CHYMOTRYPSIN TO WATER-INSOLUBLE CROSSLINKED DEXTRAN (SEPHADEX) AND SOLUBILIZATION OF ENZYME DERIVATIVES BY MEANS OF DEXTRANASE [J].
AXEN, R ;
MYRIN, PA ;
JANSON, JC .
BIOPOLYMERS, 1970, 9 (04) :401-+
[3]  
AXEN R, 1971, EUR J BIOCHEM, V18, P351
[4]   USE OF ISOCYANIDES FOR ATTACHMENT OF BIOLOGICALLY ACTIVE SUBSTANCES TO POLYMERS [J].
AXEN, R ;
VRETBLAD, P ;
PORATH, J .
ACTA CHEMICA SCANDINAVICA, 1971, 25 (03) :1129-&
[5]   JACK BEAN UREASE (EC 3.5.1.5) . DEMONSTRATION OF A CARBAMOYL-TRANSFER REACTION AND INHIBITION BY NYDROXAMIC ACIDS [J].
BLAKELEY, RL ;
HINDS, JA ;
KUNZE, HE ;
WEBB, EC ;
ZERNER, B .
BIOCHEMISTRY, 1969, 8 (05) :1991-+
[6]   COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX [J].
BROCKLEHURST, K ;
CARLSSON, J ;
KIERSTAN, MP ;
CROOK, EM .
BIOCHEMICAL JOURNAL, 1973, 133 (03) :573-584
[7]   A NOVEL REACTIVITY OF PAPAIN AND A CONVENIENT ACTIVE SITE TITRATION IN PRESENCE OF OTHER THIOLS [J].
BROCKLEHURST, K ;
LITTLE, G .
FEBS LETTERS, 1970, 9 (02) :113-+
[8]   REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS [J].
BROCKLEHURST, K ;
LITTLE, G .
BIOCHEMICAL JOURNAL, 1973, 133 (01) :67-80
[9]  
BROWN HD, 1971, CHEMISTRY CELL INT B
[10]   COMPETITIVE INHIBITION OF SEPHADEX-BOUND CHYMOTRYPSIN AND TRYPSIN [J].
CARLSSON, J ;
GABEL, D ;
AXEN, R .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1972, 353 (12) :1850-1854
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