PHOSPHOLIPASE-D ACTIVATION CAN BE NEGATIVELY REGULATED THROUGH THE ACTION OF PROTEIN-KINASE-C

被引：10

作者：

MCKINNON, M ^{[1
]}

PARKER, PJ ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,PROT PHOSPHORYLAT LAB,LONDON WC2A 3PX,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1222卷 / 01期

关键词：

PROTEIN KINASE C; PHOSPHOLIPASE D; SIGNAL TRANSDUCTION;

D O I：

10.1016/0167-4889(94)90031-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transient activation of COS-1 cell phospholipase-D (PLD) in response to the protein kinase C (PKC) agonist tetradecanoyl phorbol acetate (TPA) was demonstrated by monitoring the ethanol-dependent accumulation of phosphatidylethanol (PtdEth). Transfection of COS-1 cells with PKC-alpha (wild-type and constitutively activated mutants) produced no detectable PtdEth on incubation of transfected cells in the presence of ethanol. However, the response of transfected cells to subsequent TPA stimulation was inhibited, consistent with a role for the PKC-alpha in the suppression of PLD activity.

引用

页码：109 / 112

页数：4

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