MOLECULAR EPIDEMIOLOGY OF AN SHV-5 EXTENDED-SPECTRUM BETA-LACTAMASE IN ENTEROBACTERIACEAE ISOLATED FROM INFANTS IN A NEONATAL INTENSIVE-CARE UNIT

被引：75
作者：
VENEZIA, RA
SCARANO, FJ
PRESTON, KE
STEELE, LM
ROOT, TP
LIMBERGER, R
ARCHINAL, W
KACICA, MA
机构：
[1] ALBANY MED CTR HOSP,DEPT PATHOL & LAB MED,ALBANY,NY
[2] ALBANY MED CTR HOSP,DEPT EPIDEMIOL,ALBANY,NY
[3] ALBANY MED COLL,DEPT PEDIAT,ALBANY,NY
[4] NEW YORK STATE DEPT HLTH,WADSWORTH CTR,BACTERIOL LAB,ALBANY,NY
来源：
CLINICAL INFECTIOUS DISEASES | 1995年 / 21卷 / 04期
关键词：
D O I：
10.1093/clinids/21.4.915
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Klebsiella oxytoca that produced extended-spectrum beta-lactamase (ESBL) and were resistant to ceftazidime were isolated from infants in a neonatal intensive care unit (NICU). During a 30-week period, 3 infants developed infections and an additional 60 infants were colonized with these bacteria. The molecular typing data suggested transmission of a single strain of ceftazidime-resistant K. oxytoca among 48 of the 63 infants. The ESBL of 46 of the 48 similar isolates, 14 of the remaining 15 isolates, and 6 other Enterobacteriaceae appeared to be associated with a conjugative plasmid of similar to 85 kb. The ESBL gene was cloned, and DNA sequencing confirmed that the ESBL was an SHV-5. Hybridization data suggested that the SHV-5 gene was transmitted to other Enterobacteriaceae in vivo. The spread of the ESBL was reduced through adherence to infection-control practices.
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页码：915 / 923
页数：9
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