ASSOCIATION OF N-ETHYLMALEIMIDE-SENSITIVE FACTOR WITH SYNAPTIC VESICLES

被引：68

作者：

HONG, RM

MORI, H

FUKUI, T

MORIYAMA, Y

FUTAI, M

YAMAMOTO, A

TASHIRO, Y

TAGAYA, M

机构：

[1] OSAKA UNIV, INST SCI & IND RES, IBARAKI, OSAKA 567, JAPAN

[2] KANSAI MED UNIV, DEPT PHYSIOL, MORIGUCHI, OSAKA 570, JAPAN

来源：

FEBS LETTERS | 1994年 / 350卷 / 2-3期

基金：

美国国家科学基金会;

关键词：

SYNAPTIC VESICLE; VESICULAR TRANSPORT; NEUROTRANSMITTER;

D O I：

10.1016/0014-5793(94)00778-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Ethylmaleimide-sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi-pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti-NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca2+ influx.

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页码：253 / 257

页数：5

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