LECTIN-ACTIVATED KILLER-CELLS RAPIDLY INDUCED BY POKEWEED MITOGEN CONJUGATED BEADS AND THEIR IN-VIVO ANTITUMOR EFFECTS

被引:7
作者
OHNO, S [1 ]
TANAKA, N [1 ]
HIZUTA, A [1 ]
MIYAGI, K [1 ]
ORITA, K [1 ]
机构
[1] OKAYAMA UNIV,SCH MED,DEPT SURG 1,OKAYAMA 700,JAPAN
来源
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 1994年 / 16卷 / 09期
关键词
D O I
10.1016/0192-0561(94)90096-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pokeweed mitogen (PWM) was found to induce rapidly killer cells in human peripheral blood mononuclear cells (PBMC). To avoid PWM contamination in infused lymphocytes, PBMC were stimulated with PWM-coated beads, CMC-1, One hour of stimulation with CMC-1 led to distinct cytotoxic activity in PBMC at 7 h, this reaching a peak at 23 h in the following in vitro culture. The cytotoxic activity and target cell spectrum of CMC-1-activated killer (PWM-AK) cells were similar to those of lymphokine-activated killer (LAK) cells, the precursor cells of PWM-AK cells, as are those of LAK cells which are also low-density lymphocytes. The in vivo antitumor effects of PWM-AK cells were examined in nude mice with peritoneal carcinomatosis generated by the human colon cancer cell line, RPMI 4788. The intraperitoneal (i.p.) injection of PWM-AK cells immediately after stimulation with CMC-1 significantly prolonged the survival of tumor-bearing mice, suggesting that these cells could be of value for clinical cancer therapy.
引用
收藏
页码:761 / 768
页数:8
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