SOLUBLE CYCLIC AMP-DEPENDENT PROTEIN-KINASES FROM CHICK KIDNEY - EFFECTS OF DILUTION AND NON-PROTEIN INHIBITORS

被引：2

作者：

JONES, GM ^{[1
]}

MACNEIL, S ^{[1
]}

MARTIN, TJ ^{[1
]}

机构：

[1] UNIV SHEFFIELD,SCH MED,DEPT CHEM PATHOL,SHEFFIELD S10 2RX,S YORKSHIRE,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 582卷 / 03期

关键词：

(Chick kidney); Cyclic AMP; Protein kinase;

D O I：

10.1016/0304-4165(79)90141-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic AMP-dependent protein kinases prepared from crude cytosols of chick kidney, rat kidney and rat liver were found on dilution to exhibit complex kinetics. Dilution of the cytosols appears to increase the state of activation of the enzymes. This effect was due to the presence of inhibitory agents in the cytosol which had a greater inhibitory effect on the cyclic AMP-dependent than on the cyclic AMP-independent enzyme. Two types of inhibitory activity were found by column chromatography, one resistant to trichloroacetic acid precipitation and boiling but affected by trypsin digestion and the other resistant to boiling and trypsin digestion but precipitated by trichloroacetic acid. Inhibitory activity corresponding to the former characteristics has been described previously but the presence of additional soluble inhibitory agents in the cytosol has not been documented. The complete characterisation of this previously undescribed inhibitory activity requires further investigation. The relevance of such cytosolic inhibitory activity to the interpretation of states of activation of protein kinase enzymes is discussed. © 1979.

引用

页码：504 / 514

页数：11

共 21 条

[1] PROPERTIES OF SOLUBLE AND BOUND PROTEIN KINASES ISOLATED FROM SWINE KIDNEY [J].

ABOUISSA, H ;

KRATOWICH, N ;

MENDICINO, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 42 (02) :461-473

[2]

ASHBY CD, 1973, J BIOL CHEM, V248, P1255

[3]

Brostrom M A, 1970, Adv Enzyme Regul, V8, P191, DOI 10.1016/0065-2571(70)90017-8

[4] SIMPLE AND SENSITIVE SATURATION ASSAY METHOD FOR MEASUREMENT OF ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE [J].

BROWN, BL ;

ALBANO, JDM ;

EKINS, RP ;

SGHERZI, AM ;

TAMPION, W .

BIOCHEMICAL JOURNAL, 1971, 121 (03) :561-+

[5] NUCLEAR PROTEIN-KINASE ACTIVITY IN PERFUSED RAT-LIVER STIMULATED WITH DIBUTYRYL-ADENOSINE CYCLIC 3'-5'-MONOPHOSPHATE [J].

CASTAGNA, M ;

PALMER, WK ;

WALSH, DA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 55 (01) :193-199

[6]

CORBIN JD, 1975, J BIOL CHEM, V250, P218

[7]

CORBIN JD, 1973, J BIOL CHEM, V248, P1813

[8] STIMULATION BY LH OF CYCLIC AMP-DEPENDENT PROTEIN KINASE-ACTIVITY IN BOVINE CORPUS-LUTEUM SLICES [J].

DARBON, JM ;

URSELY, J ;

LEYMARIE, P .

FEBS LETTERS, 1976, 63 (01) :159-163

[9] HORMONAL MODULATION OF CYCLIC ADENOSINE 3', 5'-MONOPHOSPHATE-DEPENDENT PROTEIN-KINASE ACTIVITY IN RAT RENAL CORTEX - SPECIFICITY OF ENZYME TRANSLOCATION [J].

DERUBERTIS, FR ;

CRAVEN, PA .

JOURNAL OF CLINICAL INVESTIGATION, 1976, 57 (06) :1442-1450

[10] FACTORS AFFECTING BINDING OF [H-3]ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE TO PROTEIN-KINASE FROM BOVINE ADRENAL-CORTEX [J].

DOSKELAND, SO ;

UELAND, PM ;

HAGA, HJ .

BIOCHEMICAL JOURNAL, 1977, 161 (03) :653-665

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