SETS OF SHORT TANDEM REPEAT POLYMORPHISMS FOR EFFICIENT LINKAGE SCREENING OF THE HUMAN GENOME

被引：82
作者：
DUBOVSKY, J
SHEFFIELD, VC
DUYK, GM
WEBER, JL
机构：
[1] MARSHFIELD MED RES FDN,CTR GENET MED,MARSHFIELD,WI 54449
[2] UNIV IOWA,DEPT PEDIAT,IOWA CITY,IA 52242
[3] HARVARD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT GENET,BOSTON,MA 02115
来源：
HUMAN MOLECULAR GENETICS | 1995年 / 4卷 / 03期
关键词：
D O I：
10.1093/hmg/4.3.449
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
For the purpose of initial screening of the human genome in linkage mapping, two overlapping sets of high quality short tandem repeat polymorphisms (STRPs) which span the autosomes have been assembled. The higher density set contains a total of 363 markers with an average heterozygosity of 79% and an average sex-equal genetic distance between markers of 10.5 cM. The lower density set, which is a subset of the other, contains 156 markers with an average heterozygosity of 80% and an average spacing of 26.5 cM. Tri- and tetranucleotide STRPs comprised 47 and 63%, respectively, of the markers within the higher and lower density sets. Markers within the screening sets were selected to have maximum quality, where quality was defined as a blend of high informativeness, strong amplification under standard PCR conditions, low amplification background, and ease in scoring. The screening sets along with combinations of STRPs which can be amplified and electrophoresed simultaneously are available electronically through anonymous ftp.
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页码：449 / 452
页数：4
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