EARLY STIMULATION OF ALKALINE-PHOSPHATASE ACTIVITY IN RESPONSE TO "1-ALPHA,25-DIHYDROXYCHOLECALCIFEROL

被引：38
作者：
BACHELET, M
ULMANN, A
LACOUR, B
BRAMI, M
PIERANDREI, E
机构：
[1] INSERM U.90 (Pr. J.L. FUNCK-BRENTANO), Hôpital Necker -, 75015 Paris, 161, rue de Sèvres
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 02期
关键词：
D O I：
10.1016/0006-291X(79)90685-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alkaline phosphatase activity (APA) stimulation in response to 1,25-dihydroxycholecalciferol (1,25 (OH)2D3) has been studied in vitamin-D-deficient rat intestinal brush borders prepared from ex-vivo-perfused duodeno-jejunal segments. Basal APA in intestines perfused with ethanol remained constant throughout the experiments. APA was significantly increased when intestines were perfused with 1,25 (OH)2D3 (3 nM) for 30, 45 or 60 min. A dose-effect response was observed when 1,25 (OH)2D3 increased in the perfusion medium. The maximal alkaline phosphatase activity after a 45-min perfusion (2404 ± 379 mTU/mg prot.) was observed when 1,25 (OH)2D3 concentration was 6 nM. Cholecalciferol had no effect in this system. © 1979.
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页码：694 / 700
页数：7
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