SUBCELLULAR-LOCALIZATION OF CHITINASE AND OF ITS POTENTIAL SUBSTRATE IN TOMATO ROOT TISSUES INFECTED BY FUSARIUM OXYSPORUM F SP RADICIS-LYCOPERSICI

被引:128
作者
BENHAMOU, N [1 ]
JOOSTEN, MHAJ [1 ]
DEWIT, PJGM [1 ]
机构
[1] WAGENINGEN UNIV AGR, DEPT PHYTOPATHOL, 6700 EE WAGENINGEN, NETHERLANDS
关键词
D O I
10.1104/pp.92.4.1108
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Antiserum raised against a tomato (Lycopersicon esculentum Mill.) chitinase (molecular mass of 26 kilodaltons) was used as a probe to study the subcellular localization of this enzyme in tomato root tissues infected with Fusarium oxysporum f. sp. radicis-lycopersici. A time-course experiment revealed that chitinase accumulated earlier in the incompatible interaction than in the compatible one. However, in both systems, chitinase deposition was largely correlated with pathogen distribution. The enzyme was found to accumulate in areas where host walls were in close contact with fungal cells. In contrast, the enzyme could not be detected in vacuoles and intracellular spaces. The substantial amount of chitinase found at the fungus cell surface supports the view of an antifungal activity. However, the preferential association of the enzyme with altered fungal wall areas indicates that chitinase activity is either preceded by the hydrolytic action of other enzymes such as β-1,3-glucanases or coincides with these enzymes. The possibility that fungal glucans released through the action of β-1,3-glucanases may act as elicitors of chitinase production is discussed.
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页码:1108 / 1120
页数:13
相关论文
共 32 条
[1]   PREPARATION AND PURIFICATION OF GLUCANASE AND CHITINASE FROM BEAN LEAVES [J].
ABELES, FB ;
BOSSHART, RP ;
FORRENCE, LE ;
HABIG, WH .
PLANT PHYSIOLOGY, 1971, 47 (01) :129-&
[2]   HOST-PATHOGEN INTERACTIONS .8. ISOLATION OF A PATHOGEN-SYNTHESIZED FRACTION RICH IN GLUCAN THAT ELICITS A DEFENSE RESPONSE IN PATHOGENS HOST [J].
ANDERSONPROUTY, AJ ;
ALBERSHEIM, P .
PLANT PHYSIOLOGY, 1975, 56 (02) :286-291
[3]   CHITIN OLIGOSACCHARIDES ELICIT LIGNIFICATION IN WOUNDED WHEAT LEAVES [J].
BARBER, MS ;
BERTRAM, RE ;
RIDE, JP .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1989, 34 (01) :3-12
[4]   BIOCHEMICAL-MECHANISMS OF DISEASE RESISTANCE [J].
BELL, AA .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1981, 32 :21-81
[5]  
BENHAMOU N, 1989, PLANT CELL, V1, P1209
[6]  
Benhamou N, 1989, COLLOIDAL GOLD PRINC, P95
[7]   PROTEINASE INHIBITOR-INDUCING FACTOR ACTIVITY IN TOMATO LEAVES RESIDES IN OLIGOSACCHARIDES ENZYMICALLY RELEASED FROM CELL-WALLS [J].
BISHOP, PD ;
MAKUS, DJ ;
PEARCE, G ;
RYAN, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3536-3540
[8]   VACUOLAR LOCALIZATION OF ETHYLENE-INDUCED CHITINASE IN BEAN-LEAVES [J].
BOLLER, T ;
VOGELI, U .
PLANT PHYSIOLOGY, 1984, 74 (02) :442-444
[9]   COMPARISON OF SOME MOLECULAR, ENZYMATIC AND ANTIFUNGAL PROPERTIES OF CHITINASES FROM THORN-APPLE, TOBACCO AND WHEAT [J].
BROEKAERT, WF ;
VANPARIJS, J ;
ALLEN, AK ;
PEUMANS, WJ .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1988, 33 (03) :319-331
[10]  
CABIB E, 1981, PLANT CARBOHYDRATES, V2, P352