AN ENZYMATIC METHOD FOR DETERMINATION OF FORMATE PRODUCTION BY STREPTOCOCCUS-THERMOPHILUS

被引:8
作者
PEREZ, PF
DEANTONI, GL
ANON, MC
机构
[1] Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Universidad Nacional de la Plata, La Plata, 1900
关键词
enzymatic assay; Streptococcus thermophilus; yogurt;
D O I
10.3168/jds.S0022-0302(90)78954-0
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
An enzymatic determination of formate in milk samples using formate dehydrogenase (EC 1.2.1.2) was tested. The replacement of HClO4 by TCA in the deproteinization step lead to faster determinations, and no deproteinization was required for samples in culture medium. The method was applied to study formate production by Streptococcus thermophilus ATCC 19258 grown in milk and culture media. Results obtained with the method show that formate levels are higher in milk than in the culture medium. © 1990, American Dairy Science Association. All rights reserved.
引用
收藏
页码:2697 / 2701
页数:5
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