AMPEROMETRIC FLOW-INJECTION DETERMINATION OF GLUCOSE, URATE AND CHOLESTEROL IN BLOOD-SERUM BY USING SOME IMMOBILIZED ENZYME REACTORS AND A POLY(1,2-DIAMINOBENZENE)-COATED PLATINUM-ELECTRODE

被引：24

作者：

YAO, T

SATOMURA, M

NAKAHARA, T

机构：

[1] Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Sakai, Osaka, 593

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 296卷 / 03期

关键词：

AMPEROMETRY; FLOW INJECTION; ENZYME REACTOR; POLYMER-COATED ELECTRODE; GLUCOSE; URIC ACID; CHOLESTEROL;

D O I：

10.1016/0003-2670(94)80246-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose, uric acid and cholesterol in blood serum were assayed simultaneously in a flow-injection system by using a 16-way switching valve and a parallel configuration of three immobilized enzyme re actors. A poly(1,2-diaminobenzene)-coated platinum electrode was used to detect selectively hydrogen peroxide generated enzymatically into the enzyme reactors, without any interferences from oxidizable species and proteins present in serum. The precision (coefficient of variation) of the assay was better than 2% for the simultaneous assay of three species in human control serum. The assay speed was up to 38 samples per hour.

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页码：271 / 276

页数：6

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