The grain protein content of barley (Hordeum vulgare L.) is derived from the retranslocation of vegetative N, as wed as from the continued uptake and assimilation of N during grain filling. Field trials were conducted at the E.A. Lods Agronomy Research Centre of McGill University on a a Bearbrook soil (fine, mixed, nonacid, frigid Humaquept) to study the effects of fertilizer N on grain and total plant N accumulation, vegetative N retranslocation, and post-heading N uptake, and also to evaluate the importance of pre- and post-anthesis N uptake in determining grain protein concentration (GPC). 'Cadette' and 'Leger', which are adapted feed types, and 'Argyle', an unadapted malting type, were grown with N rates of 0, 50, 100, 150 and 200 kg N ha(-1) broadcast at seeding as NH4NO3. Two other treatments consisted of applying 100 kg N ha(-1) at seeding and 50 kg N ha-L as NH4NO3 (broadcast) or urea (foliar solution) 6 to 10 d after awn emergence. Retranslocation of vegetative N to the grain generally decreased with increased N application in 1988 and 1989, but not in 1990, when it increased. The proportion of vegetative N retranslocated to the grain decreased with increasing rates of fertilizer N in all years. Post-heading N uptake and assimilation increased with fertilizer N, and appeared to be the major contributor of N to the grain. Cultivar differences in GPC were not related to differences in total N uptake, grain Ei, N harvest index, and apparent post-heading N uptake or N retranslocation.