Endotoxin removal by membrane affinity chromatography

被引:0
|
作者
Guo, W
Shang, ZH
Yu, YN
Zhou, LM
Zun, ZY
Ma, LR
Chen, MY
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China
[2] Acad Mil Med Sci, Inst Radiat Med, Beijing 100850, Peoples R China
关键词
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chromatography. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand. A membrane cartridge containing 40 sheets of affinity membrane was also prepared, which can be used to remove pyrogen from aqueous solutions.
引用
收藏
页码:919 / 922
页数:4
相关论文
共 50 条
  • [1] ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY
    Wei GUO
    Zhen Hua SHANG
    Yi Nian YU and Liang Mo ZHOU(Dallan Institute of Chemical Physics.Chinese Academy of Sclences. P.O. Box 100. Dallan 116012)Zhong Yi SUN
    Li Ren MA and Mei Yun CHEN(Institute of Radiation Medicine Academy of Military Medical Scienc
    ChineseChemicalLetters, 1995, (10) : 919 - 922
  • [2] Removal of endotoxin from aqueous solutions by affinity membrane
    Guo, W
    Shang, ZH
    Yu, YN
    Zhou, LM
    BIOMEDICAL CHROMATOGRAPHY, 1997, 11 (03) : 164 - 166
  • [4] Endotoxin removal by affinity sorbents
    Anspach, FB
    JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3): : 665 - 681
  • [6] Preparation of affinity matrices for endotoxin removal
    Tang, SW
    Kong, L
    Ou, JJ
    Zou, HF
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 34 (04) : 455 - 458
  • [7] Effects of ionic strength and pH on endotoxin removal efficiency and protein recovery in an affinity chromatography
    Kang, Y
    Luo, RG
    PROCESS BIOCHEMISTRY, 2000, 36 (1-2) : 85 - 92
  • [8] Synthesis and application of a novel ligand for affinity chromatography based removal of endotoxin from antibodies
    McAllister, Laura A.
    Hixon, Mark S.
    Schwartz, Roberta
    Kubitz, Diane S.
    Janda, Kim D.
    BIOCONJUGATE CHEMISTRY, 2007, 18 (02) : 559 - 566
  • [9] Membrane adsorber for endotoxin removal
    de Almeida, Karina Moita
    Almeida, Maiara Maciel
    Fingola, Fernando Faria
    Ferraz, Helen Conceicao
    BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 52 (01) : 171 - 177
  • [10] Cellulose affinity membranes utilized for endotoxin removal
    Wei, GL
    Li, JH
    Liu, XL
    Shang, ZH
    Yu, YN
    Wang, JD
    Pan, MC
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2001, 29 (10) : 1117 - 1120