COMMERCIAL-SCALE REFOLDING OF RECOMBINANT METHIONYL BOVINE SOMATOTROPIN

被引：0

作者：

STORRS, SB

PRZYBYCIEN, TM

机构：

来源：

ACS SYMPOSIUM SERIES | 1991年 / 470卷

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中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method to dissolve and oxidize recombinant methionyl bovine somatotropin (mBST) from bacterial inclusion bodies suitable for large scale manufacture has been developed. mBST-containing inclusion bodies are dissolved, refolded, and oxidized in a single step in 4.5 M urea and pH 11.25 at protein concentrations in the 5 - 15 g/L range with yields in excess of 80%. Yield is a strong function of the urea concentration used during the oxidation reaction; optimal yields are obtained at intermediate urea concentrations.

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页码：197 / 205

页数：9

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