EFFICIENT INCORPORATION OF GALACTOSE INTO LIPOPOLYSACCHARIDE BY ESCHERICHIA-COLI K-12 STRAINS WITH POLAR GALE MUTATIONS

被引：13

作者：

SCHNAITMAN, CA ^{[1
]}

AUSTIN, EA ^{[1
]}

机构：

[1] UNIV VIRGINIA,SCH MED,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 09期

关键词：

D O I：

10.1128/jb.172.9.5511-5513.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.

引用

页码：5511 / 5513

页数：3

共 17 条

[1]

ADHYA S, 1987, ESCHERICHIA COLI SAL, V2, P1503

[2] GENETIC-ANALYSIS OF LIPOPOLYSACCHARIDE CORE BIOSYNTHESIS BY ESCHERICHIA-COLI K-12 - INSERTION MUTAGENESIS OF THE RFA LOCUS [J].

AUSTIN, EA ;

GRAVES, JF ;

HITE, LA ;

PARKER, CT ;

SCHNAITMAN, CA .

JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5312-5325

[3]

BOCHNER BR, 1982, J BIOL CHEM, V257, P9759

[4] INTERACTION OF SPATIALLY SEPARATED PROTEIN DNA COMPLEXES FOR CONTROL OF GENE-EXPRESSION - OPERATOR CONVERSIONS [J].

HABER, R ;

ADHYA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9683-9687

[5] MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].

HITCHCOCK, PJ ;

BROWN, TM .

JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :269-277

[6] AN EQUILIBRIUM BETWEEN 2 FRACTIONS OF LIPOPOLYSACCHARIDE IN ESCHERICHIA COLI [J].

LEVY, SB ;

LEIVE, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 61 (04) :1435-&

[7]

Miller J.H., 1972, EXPT MOL GENETICS, P1

[8] AN INTERMEDIATE STEP IN TRANSLOCATION OF LIPOPOLYSACCHARIDE TO THE OUTER-MEMBRANE OF SALMONELLA-TYPHIMURIUM [J].

MULFORD, CA ;

OSBORN, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1159-1163

[9] STUDIES ON BIOSYNTHESIS OF CELL WALL POLYSACCHARIDE IN MUTANT STRAINS OF SALMONELLA, II [J].

NIKAIDO, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (09) :1542-&

[10] BIOSYNTHESIS OF BACTERIAL LIPOPOLYSACCHARIDE .1. ENZYMATIC INCORPORATION OF GALACTOSE IN A MUTANT STRAIN OF SALMONELLA [J].

OSBORN, MJ ;

HORECKER, BL ;

ROTHFIELD, L ;

ROSEN, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (10) :1831-&

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