FACTOR REQUIRED FOR MAMMALIAN SPLICEOSOME ASSEMBLY IS LOCALIZED TO DISCRETE REGIONS IN THE NUCLEUS

被引：661
作者：
FU, XD
MANIATIS, T
机构：
[1] Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
来源：
NATURE | 1990年 / 343卷 / 6257期
关键词：
D O I：
10.1038/343437a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus. © 1990 Nature Publishing Group.
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页码：437 / 441
页数：5
相关论文
共 42 条
[1]   IDENTIFICATION OF A FUNCTIONAL MAMMALIAN SPLICEOSOME CONTAINING UNSPLICED PRE-MESSENGER RNA [J].
ABMAYR, SM ;
REED, R ;
MANIATIS, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7216-7220
[2]   SPLICE SITE SELECTION, RATE OF SPLICING, AND ALTERNATIVE SPLICING ON NASCENT TRANSCRIPTS [J].
BEYER, AL ;
OSHEIM, YN .
GENES & DEVELOPMENT, 1988, 2 (06) :754-765
[3]   AN ORDERED PATHWAY OF SNRNP BINDING DURING MAMMALIAN PRE-MESSENGER-RNA SPLICING COMPLEX ASSEMBLY [J].
BINDEREIF, A ;
GREEN, MR .
EMBO JOURNAL, 1987, 6 (08) :2415-2424
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   THE 3' SPLICE SITE OF PRE-MESSENGER RNA IS RECOGNIZED BY A SMALL NUCLEAR RIBONUCLEOPROTEIN [J].
CHABOT, B ;
BLACK, DL ;
LEMASTER, DM ;
STEITZ, JA .
SCIENCE, 1985, 230 (4732) :1344-1349
[6]   RNA11 PROTEIN IS ASSOCIATED WITH THE YEAST SPLICEOSOME AND IS LOCALIZED IN THE PERIPHERY OF THE CELL-NUCLEUS [J].
CHANG, TH ;
CLARK, MW ;
LUSTIG, AJ ;
CUSICK, ME ;
ABELSON, J .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2379-2393
[7]   RIBONUCLEIC-ACID PRECURSORS ARE ASSOCIATED WITH THE CHICK OVIDUCT NUCLEAR MATRIX [J].
CIEJEK, EM ;
NORDSTROM, JL ;
TSAI, MJ ;
OMALLEY, BW .
BIOCHEMISTRY, 1982, 21 (20) :4945-4953
[8]  
DENG JS, 1981, J CELL BIOL, V91, P572
[9]   ULTRASTRUCTURAL DISTRIBUTION OF NUCLEAR RIBONUCLEOPROTEINS AS VISUALIZED BY IMMUNOCYTOCHEMISTRY ON THIN-SECTIONS [J].
FAKAN, S ;
LESER, G ;
MARTIN, TE .
JOURNAL OF CELL BIOLOGY, 1984, 98 (01) :358-363
[10]   STEPWISE ASSEMBLY OF A PRE-MESSENGER RNA SPLICING COMPLEX REQUIRES U-SNRNPS AND SPECIFIC INTRON SEQUENCES [J].
FRENDEWEY, D ;
KELLER, W .
CELL, 1985, 42 (01) :355-367
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