THE GENE DIS2S1 IS ESSENTIAL IN SACCHAROMYCES-CEREVISIAE AND IS INVOLVED IN GLYCOGEN-PHOSPHORYLASE ACTIVATION

被引：34

作者：

CLOTET, J ^{[1
]}

POSAS, F ^{[1
]}

CASAMAYOR, A ^{[1
]}

SCHAAFFGERSTENSCHLAGER, I ^{[1
]}

ARINO, J ^{[1
]}

机构：

[1] TH DARMSTADT,INST MIKROBIOL,W-6100 DARMSTADT,GERMANY

来源：

CURRENT GENETICS | 1991年 / 19卷 / 05期

关键词：

GENE DISRUPTION; PROTEIN PHOSPHATASES; GLYCOGEN PHOSPHORYLASE;

D O I：

10.1007/BF00309593

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells.

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页码：339 / 342

页数：4

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