SEPARATION OF DNA FRAGMENTS BY HIGH-RESOLUTION ION-EXCHANGE CHROMATOGRAPHY ON A NONPOROUS QA COLUMN

被引：12

作者：

OHMIYA, Y ^{[1
]}

KONDO, Y ^{[1
]}

KONDO, T ^{[1
]}

机构：

[1] GUNMA UNIV,INST ENDOCRINOL,DEPT PHYS BIOCHEM,MAEBASHI,GUNMA 371,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 189卷 / 01期

关键词：

Ion exchange;

D O I：

10.1016/0003-2697(90)90057-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nonporous QA column (a strong anion exchanger) was used for HPLC of DNA fragments. This column was successfully employed to separate small (ca. 10 bp) and intermediate size (ca. 10 kbp) DNA fragments from each other. The column also separated double-stranded DNA from its single-stranded form, and circular DNA molecules from linear ones. The entire separation process was completed within 60 min. The recovery of DNA fragments in each run was above 95%. High resolution was obtained both at an analytical level (microgram scale) and at a preparative level (100 μg scale). In view of time efficiency, recovery, and resolution, the nonporous QA column is superior to other porous ion-exchange columns and expected to be a very useful tool in molecular biological studies. © 1990.

引用

页码：126 / 130

页数：5

共 22 条

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