A TECHNIQUE FOR THE MEASUREMENT OF TOTAL AMMONIA IN SMALL VOLUMES OF SEAWATER AND HEMOLYMPH

被引：48

作者：

HUNTER, DA ^{[1
]}

UGLOW, RF ^{[1
]}

机构：

[1] UNIV HULL,DEPT APPL BIOL,HULL HU6 7RX,N HUMBERSIDE,ENGLAND

来源：

OPHELIA | 1993年 / 37卷 / 01期

关键词：

AMMONIA; SEAWATER; HEMOLYMPH;

D O I：

10.1080/00785326.1993.10430375

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

A rapid and precise technique combining flow-injection analysis and gas-diffusion is described for the measurement of total ammonia (NH3 + NH4+) in small volumes (100-450 mul) of seawater and diluted haemolymph. Calibration of the method with seawater ammonia standard solutions in the range 1.0-50.0 mumol ammonia l-1 (n = 8), and with saline ammonia standard solutions in the range 5.0-50.0 mumol ammonia l-1 (n = 6) gave linear calibration curves (r = 0.999 in each case) with precision in the range 0.2-3.0%. Precision of measurement of ammonia in samples of seawater and diluted haemolymph was 0.9-3.3% (n = 11-13). The lower limit of detection is 0.20 mumol ammonia l-1. Thirty determinations can be made per hour.

引用

页码：31 / 40

页数：10

共 21 条

[1]

ARNOLD MA, 1983, ANAL CHIM ACTA, V153, P33

[2]

BINNS R, 1969, J EXP BIOL, V51, P41

[3] AN AUTOMATED SPECTROPHOTOMETRIC FIELD-MONITOR FOR WATER-QUALITY PARAMETERS - DETERMINATION OF AMMONIA [J].

CLINCH, JR ;

WORSFOLD, PJ ;

SWEETING, FW .

ANALYTICA CHIMICA ACTA, 1988, 214 (1-2) :401-407

[4]

CLINCH JR, 1988, THESIS U HULL

[5]

DAGG M J, 1976, Internationale Revue der Gesamten Hydrobiologie, V61, P297, DOI 10.1002/iroh.19760610303

[6] MICRO-FLUOROMETRIC METHOD TO MEASURE AMMONIUM IN NATURAL-WATERS [J].

GARDNER, WS .

LIMNOLOGY AND OCEANOGRAPHY, 1978, 23 (05) :1069-1072

[7] DETERMINATION OF SUBMICROMOLAR CONCENTRATIONS OF AMMONIA IN NATURAL-WATERS BY A STANDARD ADDITION METHOD USING A GAS-SENSING ELECTRODE [J].

GARSIDE, C ;

HULL, G ;

MURRAY, S .

LIMNOLOGY AND OCEANOGRAPHY, 1978, 23 (05) :1073-1076

[8] DETERMINATION OF AMMONIA IN AQUARIA AND IN SEA-WATER USING AMMONIA ELECTRODE [J].

GILBERT, TR ;

CLAY, AM .

ANALYTICAL CHEMISTRY, 1973, 45 (09) :1757-1759

[9] ASPECTS OF BLOOD PHYSIOLOGY AND AMMONIA EXCRETION IN NEPHROPS-NORVEGICUS UNDER HYPOXIA [J].

HAGERMAN, L ;

SONDERGAARD, T ;

WEILE, K ;

HOSIE, D ;

UGLOW, RF .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1990, 97 (01) :51-55

[10] DETERMINATION OF NITRATE AND NITRITE IN SEAWATER BY FLOW-INJECTION ANALYSIS [J].

JOHNSON, KS ;

PETTY, RL .

LIMNOLOGY AND OCEANOGRAPHY, 1983, 28 (06) :1260-1266

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