TOWARDS AUTOMATIC ELECTRON TOMOGRAPHY .2. IMPLEMENTATION OF AUTOFOCUS AND LOW-DOSE PROCEDURES

被引:101
作者
DIERKSEN, K
TYPKE, D
HEGERL, R
BAUMEISTER, W
机构
[1] Max-Planck-Institut für Biochemie
关键词
D O I
10.1016/0304-3991(93)90217-L
中图分类号
TH742 [显微镜];
学科分类号
摘要
Automatic electron tomography involves the compensation for lateral specimen displacements and, at higher resolution, refocusing. Improved automatic procedures are described and the accuracy and reliability of the basic steps is assessed. It is demonstrated that the cumulative dose in collecting tomographic 3D data sets can be kept small enough for the investigation of ice-embedded specimens.
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页码:109 / 120
页数:12
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