REVERSE MICELLE EXTRACTION OF LIPASE USING AOT AND TAURODEOXYCHOLIC ACID

Forward and backward liquid-liquid extraction of Chromobacterium viscosum lipase were successfully carried out using reverse micellar systems. The reverse micellar system prepared by dissolving both AOT and taurodeoxycholic acid (TDCA) in isooctane was found to form a stable mixed micelle showing distinct advantages in both activity yield and selectivity for lipase over the conventional AOT reverse micellar system. The sizes of mixed micelles measured by using a dynamic light-scattering method were not different from those of AOT micelles (50mM AOT) and did not vary with TDCA concentration in the range of 0-4 mM. Nearly 6.2 molecules of AOT were replaced with a TDCA molecule at the micellar interface. The partition coefficient of lipase was selectively improved up to eight-fold in contrast to alpha-chymotrypsin and papain. Its activity yield was also improved by using a simple phase transfer or liquid membrane extraction method.