INCREASE IN DATP POOL IN APHIDICOLIN-RESISTANT MUTANTS OF MOUSE FM3A CELLS

被引：33

作者：

AYUSAWA, D ^{[1
]}

IWATA, K ^{[1
]}

KOZU, T ^{[1
]}

IKEGAMI, S ^{[1
]}

SENO, T ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91971-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutants that were resistant to aphidicolin were isolated from mutagenized mouse FM3A cells at a frequency of about 10-6. Resistance to aphidicolin in these mutants was not due to an effect on [3H]thymidine incorporation into DNA, DNA synthesis in permeabilized cells, or DNA polymerase α. All the mutants showed a greatly increased dATP pool and decreased ability to incorporate [3H]deoxycytidine into DNA. They also showed cross-resistance to both 1-β-D-arabinofuranosyladenine and 1-β-D-arabinofuranosylcytosine. These results indicate that an enzyme involved in production of dATP or its regulation is altered in these mutants. It is suggested that dATP competes with aphidocolin at its killing site or that dATP reverses the effect of aphidicolin by some unknown mechanism in vivo. © 1979.

引用

页码：946 / 954

页数：9

共 22 条

[1]

ALBRECHT AM, 1972, CANCER RES, V32, P1539

[2] APHIDICOLIN INHIBITS EUKARYOTIC DNA-REPLICATION AND REPAIR - IMPLICATIONS FOR INVOLVEMENT OF DNA POLYMERASE-ALPHA IN BOTH PROCESSES [J].