EXPRESSION OF PHOTOSYNTHESIS GENE-PROMOTER FUSIONS IN LEAF EPIDERMAL-CELLS OF TRANSGENIC TOBACCO PLANTS

被引：39
|
作者：
DUPREE, P [1 ]
PWEE, KH [1 ]
GRAY, JC [1 ]
机构：
[1] UNIV CAMBRIDGE,SCH BOT,DOWNING ST,CAMBRIDGE CB2 3EA,ENGLAND
来源：
PLANT JOURNAL | 1991年 / 1卷 / 01期
关键词：
D O I：
10.1111/j.1365-313X.1991.00115.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Fusions of the promoter regions of the pea plastocyanin, pea ferredoxin:NADP+ reductase and tobacco rbcS genes to the beta-glucuronidase (GUS) reporter gene have been introduced into tobacco via Agro-bacterium-mediated transformation, and epidermal peels of the lower leaf surface of tissue-cultured and greenhouse-grown plants examined histochemically for GUS activity. For each of the constructs, GUS was detected in epidermal cells as well as in stomatal guard cells. Epidermal peels from plants in tissue culture stained more readily than those from green-house-grown plants. Light and electron microscopy clearly demonstrated the presence of chloroplasts in epidermal cells of tobacco leaves. These results provide further evidence for the correlation between the presence of chloroplasts and the expression of nuclear genes for photosynthesis components.
引用
收藏
页码：115 / 120
页数：6
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