PREPARATION OF EUROPIUM-LABELED DERIVATIVES OF CORTISOL FOR TIME-RESOLVED FLUOROIMMUNOASSAYS

被引：24
作者：
MIKOLA, H
MIETTINEN, P
机构：
[1] Wallac Chemical Laboratories, Turku
来源：
STEROIDS | 1991年 / 56卷 / 01期
关键词：
STEROIDS; CORTISOL; TIME-RESOLVED FLUOROIMMUNOASSAY; EUROPIUM; NONRADIOACTIVE LABELING;
D O I：
10.1016/0039-128X(91)90109-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cortisol 3-(O-carboxymethyl)oxime, 6- and 21-hemisuccinoxycortisol, and cortisol 7-carboxyethyl thioether were synthesized. These carboxyl derivatives were labeled using a described general labeling method with an europium chelate. The labeled steroids were tested in a competitive time-resolved fluoroimmunoassay using antibodies raised against cortisol. Only the site-homologous antigen-antibody pairs underwent immunoreaction and gave satisfactory calibration curves.
引用
收藏
页码：17 / 21
页数：5
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