ACTIVATION OF TRANSCRIPTION FACTORS BY INTERFERON-ALPHA IN A CELL-FREE SYSTEM

被引：69

作者：

DAVID, M ^{[1
]}

LARNER, AC ^{[1
]}

机构：

[1] CTR BIOL EVALUAT & RES, DIV CYTOKINE BIOL, BETHESDA, MD 20892 USA

来源：

SCIENCE | 1992年 / 257卷 / 5071期

关键词：

D O I：

10.1126/science.1496402

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The signal transduction mechanisms of interferons (IFNs) remain unclear partly because no effect of IFN has been reproducible in a cell-free system. IFN-alpha rapidly induces the transcription of a set of early response genes, and a multicomponent transcriptional activator, interferon stimulated gene factor 3 (ISGF3), is activated within minutes after binding of IFN-alpha to its receptor. A system was developed in which IFN-alpha activated ISGF3 in homogenates of HeLa cells. Subcellular fractionation revealed that incubation of a plasma membrane-enriched fraction with IFN-alpha was sufficient to activate the regulatory subcomponent of ISGF3.

引用

页码：813 / 815

页数：3

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