NEW DERIVATIVES FOR ELECTRON CAPTURE-GAS CHROMATOGRAPHY OF STEROIDS - A SIMPLIFIED PROCEDURE FOR MEASURING PLASMA TESTOSTERONE

被引：23
作者：
KRISCHNE.MA
TAYLOR, JP
机构：
[1] Endocrinology Branch, National Cancer Institute, National Institutes of Health, Bethesda
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 03期
关键词：
D O I：
10.1016/0003-2697(69)90127-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two new derivatives are described that confer electron-capturing properties to hydroxyl-containing steroids. The 9-H-hexadecafluoronanoate (HFN) and 11-H-eicosafluoroundecanoate (EFU) derivative of testosterone have 2.30 and 2.55 times the area response of equimolar amounts of testosterone heptafluorobutyrate, as well as longer retention times on both polar and nonpolar phases. Thin-layer and GLC properties of the 17-mono-derivatives and the 3,17-di-derivatives of estradiol are presented. Using the HFN derivative, a simplified method is presented for the measurement of testosterone in human plasma. © 1969.
引用
收藏
页码：346 / &
相关论文
共 14 条
[1]   DETERMINATION OF FREE ESTRONE IN BLOOD PLASMA BY GAS-PHASE CHROMATOGRAPHY WITH ELECTRON CAPTURE DETECTION [J].
ATTAL, J ;
HENDELES, SM ;
EIKNES, KB .
ANALYTICAL BIOCHEMISTRY, 1967, 20 (03) :394-&
[2]   DETERMINATION OF TESTOSTERONE IN HUMAN PERIPHERAL BLOOD USING GAS-LIQUID CHROMATOGRAPHY WITH ELECTRON CAPTURE DETECTION [J].
BROWNIE, AC ;
VANDERMOLEN, HJ ;
EIKNES, KB ;
NISHIZAWA, EE .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1964, 24 (11) :1091-+
[3]   SEPARATION AND DETECTION OF NANOGRAM AMOUNTS OF STEROIDS [J].
CLARK, SJ ;
WOTIZ, HH .
STEROIDS, 1963, 2 (05) :535-540
[4]   ULTRAMICRO DETERMINATION OF PLASMA TESTOSTERONE BY ELECTRON-CAPTURE DETECTION OF TESTOSTERONE DIHEPTAFLUOROBUTYRATE [J].
EXLEY, D .
BIOCHEMICAL JOURNAL, 1968, 107 (02) :285-&
[5]   PROPERTIES OF STEROIDAL 3 ENOL HEPTAFLUOROBUTYRATES [J].
EXLEY, D ;
CHAMBERLAIN, J .
STEROIDS, 1967, 10 (05) :509-+
[6]  
EXLEY D, 1966, EXCERPTA MED INT 101, P11
[7]  
HORTON R, 1967, STEROIDS, V10, P257
[8]  
KRISCHNER MA, 1968, J CLIN ENDOCRINOL, V28, P1347
[9]   DETERMINATION OF PLASMA TESTOSTERONE BY USE OF COMPETITIVE PROTEIN BINDING [J].
MAYES, D ;
NUGENT, CA .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1968, 28 (08) :1169-+
[10]   DETERMINATION OF TESTOSTERONE BY GAS-LIQUID CHROMATOGRAPHY USING AN ELECTRON CAPTURE DETECTOR .I. RESPONSES OF HALO-ALKYL DERIVATIVES [J].
NAKAGAWA, K ;
MCNIVEN, NL ;
FORCHIEL.E ;
VERMEULE.A ;
DORFMAN, RI .
STEROIDS, 1966, 7 (04) :329-&
12