MEASUREMENT OF PROTEIN USING FLOW-INJECTION ANALYSIS WITH BICINCHONINIC ACID

被引：30
作者：
DAVIS, LC
RADKE, GA
机构：
[1] Department of Biochemistry, Kansas State University, Manhattan
来源：
ANALYTICAL BIOCHEMISTRY | 1987年 / 161卷 / 01期
关键词：
bicinchoninic acid; flow injection analysis;
D O I：
10.1016/0003-2697(87)90665-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used the bicinchoninic acid reagent developed by Pierce Chemical Co. to measure proteins in a simple flow injection analyzer. The sensitivity is comparable to that of the Lowry method and no pipetting of reagents is needed. Results are obtained in less than 1 min and samples may be run at a rate of 60/h. The response is linear over a range of protein concentration (0-10 μg) and sample size (5-20 μl) convenient for most analytical requirements. A peristaltic pump, a controlled-temperature water bath, and a spectrophotometer with flow cuvette are the only special apparatus required. © 1987.
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收藏
页码：152 / 156
页数：5
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