IDENTIFICATION AND PURIFICATION OF HUMAN STAT PROTEINS ACTIVATED IN RESPONSE TO INTERLEUKIN-2

被引：209

作者：

HOU, JZ ^{[1
]}

SCHINDLER, U ^{[1
]}

HENZEL, WJ ^{[1
]}

WONG, SC ^{[1
]}

MCKNIGHT, SL ^{[1
]}

机构：

[1] GENENTECH INC,S SAN FRANCISCO,CA 94080

来源：

IMMUNITY | 1995年 / 2卷 / 04期

关键词：

D O I：

10.1016/1074-7613(95)90140-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A key cytokine induced during the immune response is IL-2. Following T cell activation, the genes encoding IL-2 and the various chains of its receptor are transcriptionally induced. In turn, secreted IL-2 serves to stimulate the proliferation and differentiation of T lymphocytes. Several recent studies have implicated Jak kinases in the signaling pathway induced by IL-2. Following this lead, we set out to identify transcription factors induced in response to IL-2. Human peripheral blood lymphocytes were observed to contain several IL-2-inducible DNA binding activities. Similar activities were also observed in a transformed human lymphocyte line, termed YT. We have purified these activities and found that the principal IL-2-inducible component bears significant relatedness to a prolactin-induced transcription factor first identified in sheep mammary gland tissue. We hypothesize that activation of this protein, designated hStat5, helps govern the biological effects of IL-2 during the immune response.

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页码：321 / 329

页数：9

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