CELL-GROWTH ON IMMOBILIZED CELL-GROWTH FACTOR .2. ADHESION AND GROWTH OF FIBROBLAST CELLS ON POLY(METHYL METHACRYLATE) MEMBRANE IMMOBILIZED WITH PROTEINS OF VARIOUS KINDS

被引：36

作者：

ITO, Y ^{[1
]}

LIU, SQ ^{[1
]}

NAKABAYASHI, M ^{[1
]}

IMANISHI, Y ^{[1
]}

机构：

[1] KYOTO UNIV,DEPT POLYMER CHEM,YOSHIDA HONMACHI,SAKYO KU,KYOTO 60601,JAPAN

来源：

BIOMATERIALS | 1992年 / 13卷 / 11期

关键词：

CELL GROWTH; CELL ADHESION; IMMOBILIZATION; POLY(METHYL METHACRYLATE); FIBROBLASTS;

D O I：

10.1016/0142-9612(92)90019-K

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The surface of poly(methyl methacrylate) membrane was partially hydrolysed and the carboxyl groups produced were coupled with various protein molecules with water-soluble carbodiimide. The immobilized proteins were a cell-growth factor insulin, cell adhesion factors fibrinogen and fibronectin, and serum proteins albumin and gamma-globulin. The insulin-immobilized poly(methyl methacrylate) membrane strongly accelerated the growth and slightly accelerated the adhesion of fibroblast cells. The immobilized fibronectin and fibrinogen enhanced the cell adhesion, and the former also accelerated the cell growth. The immobilized albumin and gamma-globulin influenced the adhesion and growth of cells very little. It was found that various proteins specifically influence the adhesion and growth of cells in an immobilized state.

引用

页码：789 / 794

页数：6

共 21 条

[11] ENHANCEMENT OF CELL-GROWTH ON GROWTH FACTOR-IMMOBILIZED POLYMER FILM [J].

ITO, Y ;

LIU, SQ ;

IMANISHI, Y .

BIOMATERIALS, 1991, 12 (05) :449-453

[12]

ITO Y, 1990, POLYM PREP JPN, V39, P2541

[13] FIBRONECTIN ENHANCES EARLY SHEAR-STRESS RESISTANCE OF SEEDED ADULT HUMAN VENOUS ENDOTHELIAL-CELLS [J].

IVARSSON, BL ;

CAMBRIA, RP ;

MEGERMAN, J ;

ABBOTT, WM .

JOURNAL OF SURGICAL RESEARCH, 1989, 47 (03) :203-207

[14]

LIU SQ, UNPUB J BIOL CHEM

[15]

LIU SQ, 1989, BIOMATERIALS, V13, P50

[16]

LIU SQ, UNPUB ENZYME MICROB

[17] CELLULAR INTERACTIONS WITH SYNTHETIC-POLYMER SURFACES IN CULTURE [J].

LYDON, MJ ;

MINETT, TW ;

TIGHE, BJ .

BIOMATERIALS, 1985, 6 (06) :396-402

[18] DYNAMIC CYTOSKELETON INTEGRIN ASSOCIATIONS INDUCED BY CELL BINDING TO IMMOBILIZED FIBRONECTIN [J].

MUELLER, SC ;

KELLY, T ;

DAI, MZ ;

DAI, HN ;

CHEN, WT .

JOURNAL OF CELL BIOLOGY, 1989, 109 (06) :3455-3464

[19] PLASMA MODIFICATION AND COLLAGEN BINDING TO PTFE GRAFTS [J].

TRAN, CNB ;

WALT, DR .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1989, 132 (02) :373-381

[20]

UNDERWOOD PA, 1989, J CELL SCI, V93, P641

← 1 2 3 →