Phenotypic characterization of an Arabidopsis T-DNA insertion line SALK_063500

被引：0

作者：

Sng, Natasha J. ^{[1
]}

Paul, Anna-Lisa ^{[1
,2
]}

Ferl, Robert J. ^{[1
,2
,3
]}

机构：

[1] Univ Florida, Plant Mol & Cellular Biol, Fifield Hall,2550 Hull Rd, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Hort Sci, Fifield Hall,2550 Hull Rd, Gainesville, FL 32611 USA

[3] Univ Florida, ICBR, 2033 Mowry Rd, Gainesville, FL 32601 USA

来源：

DATA IN BRIEF | 2018年 / 18卷

关键词：

Arabidopsis; T-DNA; SALK_063500; Silique; Pollen; Phenotype; AT1G05290;

D O I：

10.1016/j.dib.2018.03.107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this article we report the identification of a homozygous lethal T-DNA (transfer DNA) line within the coding region of the At1G05290 gene in the genome of Arabidopsis thaliana (Arabidopsis) line, SALK_063500. The T-DNA insertion is found within exon one of the AT1G05290 gene, however a homozygous T-DNA allele is unattainable. In the heterozygous T-DNA allele the expression levels of AT1G05290 were compared to wild type Arabidopsis (Col-0, Columbia). Further analyses revealed an aberrant silique phenotype found in the heterozygous SALK_063500 plants that is attributed to the reduced rate of pollen tube germination. These data are original and have not been published elsewhere. (C) 2018 The Authors. Published by Elsevier Inc.

引用

页码：913 / 919

页数：7

共 6 条

[1] The DEFECTIVE IN ANTHER DEHISCENCE1 gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis [J].

Ishiguro, S ;

Kawai-Oda, A ;

Ueda, J ;

Nishida, I ;

Okada, K .

PLANT CELL, 2001, 13 (10) :2191-2209

[2] The Arabidopsis Information Resource (TAIR): improved gene annotation and new tools [J].

Lamesch, Philippe ;

Berardini, Tanya Z. ;

Li, Donghui ;

Swarbreck, David ;

Wilks, Christopher ;

Sasidharan, Rajkumar ;

Muller, Robert ;

Dreher, Kate ;

Alexander, Debbie L. ;

Garcia-Hernandez, Margarita ;

Karthikeyan, Athikkattuvalasu S. ;

Lee, Cynthia H. ;

Nelson, William D. ;

Ploetz, Larry ;

Singh, Shanker ;

Wensel, April ;

Huala, Eva .

NUCLEIC ACIDS RESEARCH, 2012, 40 (D1) :D1202-D1210

[3] Control of pollen tube tip growth by a pop GTPase-dependent pathway that leads to tip-localized calcium influx [J].

Li, H ;

Lin, YK ;

Heath, RM ;

Zhu, MX ;

Yang, ZB .

PLANT CELL, 1999, 11 (09) :1731-1742

[4] Transgene expression patterns indicate that spaceflight affects stress signal perception and transduction in Arabidopsis [J].

Paul, AL ;

Daugherty, CJ ;

Bihn, EA ;

Chapman, DK ;

Norwood, KLL ;

Ferl, RJ .

PLANT PHYSIOLOGY, 2001, 126 (02) :613-621

[5] NIH Image to ImageJ: 25 years of image analysis [J].

Schneider, Caroline A. ;

Rasband, Wayne S. ;

Eliceiri, Kevin W. .

NATURE METHODS, 2012, 9 (07) :671-675

[6]

Sng N., 2014, GRAVIT SPACE RES, V2, P2

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