RECOMBINANT ANTIBODY-ALKALINE PHOSPHATASE CONJUGATES FOR DIAGNOSIS OF HUMAN IGGS - APPLICATION TO ANTI-HBSAG DETECTION

被引：50

作者：

CARRIER, A ^{[1
]}

DUCANCEL, F ^{[1
]}

SETTIAWAN, NB ^{[1
]}

CATTOLICO, L ^{[1
]}

MAILLERE, B ^{[1
]}

LEONETTI, M ^{[1
]}

DREVET, P ^{[1
]}

MENEZ, A ^{[1
]}

BOULAIN, JC ^{[1
]}

机构：

[1] CEA SACLAY,DEPT INGN & ETUD PROT,F-91191 GIF SUR YVETTE,FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 181卷 / 02期

关键词：

ALKALINE PHOSPHATASE; IMMUNOASSAY; RECOMBINANT TRACER; COLORIMETRIC ANTIBODY; ANTI-HBSAG DETECTION;

D O I：

10.1016/0022-1759(94)00344-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have designed an expression vector permitting the production in the periplasm of Escherichia coli of a fusion protein comprising a dimer of bacterial alkaline phosphatase (PhoA) and two Fab or scFv fragments of a monoclonal antibody directed against human IgG. Each hybrid protein expressed both high specificity for the antigen and full PhoA activity. We show that crude periplasmic extracts containing these conjugates can be used as such in enzyme immunoassays for the detection of human IgG, as exemplified in the case of anti-hepatitis B immunoglobulin.

引用

页码：177 / 186

页数：10

共 25 条

[1]

APPLEBURY ML, 1969, J BIOL CHEM, V244, P308

[2]

AVRAMEAS S, 1978, SCAND J IMMUNOL S7, V8, P7

[3]

COUROUCEPAUTY AM, 1978, TRANSPLANTATION CLIN

[4] SINGLE CHAIN ANTIBODY (SCA) ENCODING GENES - ONE-STEP CONSTRUCTION AND EXPRESSION IN EUKARYOTIC CELLS [J].

DAVIS, GT ;

BEDZYK, WD ;

VOSS, EW ;

JACOBS, TW .

BIO-TECHNOLOGY, 1991, 9 (02) :165-169

[5] RECOMBINANT COLORIMETRIC ANTIBODIES - CONSTRUCTION AND CHARACTERIZATION OF A BIFUNCTIONAL F(AB)2/ALKALINE PHOSPHATASE CONJUGATE PRODUCED IN ESCHERICHIA-COLI [J].

DUCANCEL, F ;

GILLET, D ;

CARRIER, A ;

LAJEUNESSE, E ;

MENEZ, A ;

BOULAIN, JC .

BIO-TECHNOLOGY, 1993, 11 (05) :601-605

[6]

DUCANCEL F, 1992, CR ACAD SCI III-VIE, V315, P221

[7] ENZYME-IMMUNOASSAY USING A RAT PROLACTIN ALKALINE-PHOSPHATASE RECOMBINANT TRACER [J].

GILLET, D ;

EZAN, E ;

DUCANCEL, F ;

GAILLARD, C ;

ARDOUIN, T ;

ISTIN, M ;

MENEZ, A ;

BOULAIN, JC ;

GROGNET, JM .

ANALYTICAL CHEMISTRY, 1993, 65 (13) :1779-1784

[8] INSERTION OF A DISULFIDE-CONTAINING NEUROTOXIN INTO ESCHERICHIA-COLI ALKALINE-PHOSPHATASE - THE HYBRID RETAINS BOTH BIOLOGICAL-ACTIVITIES [J].

GILLET, D ;

DUCANCEL, F ;

PRADEL, E ;

LEONETTI, M ;

MENEZ, A ;

BOULAIN, JC .

PROTEIN ENGINEERING, 1992, 5 (03) :273-278

[9]

GRASSI J, 1987, HDB EXPT PHARM, V82, P91

[10] PROCESSING BY OMPT OF FUSION PROTEINS CARRYING THE HLYA TRANSPORT SIGNAL DURING SECRETION BY THE ESCHERICHIA-COLI HEMOLYSIN TRANSPORT-SYSTEM [J].

HANKE, C ;

HESS, J ;

SCHUMACHER, G ;

GOEBEL, W .

MOLECULAR AND GENERAL GENETICS, 1992, 233 (1-2) :42-48

← 1 2 3 →