INHIBITION OF MYELOID DIFFERENTIATION BY THE HELIX-LOOP-HELIX PROTEIN ID

被引：242
作者：
KREIDER, BL
BENEZRA, R
ROVERA, G
KADESCH, T
机构：
[1] UNIV PENN,SCH MED,HOWARD HUGHES MED INST,PHILADELPHIA,PA 19104
[2] UNIV PENN,SCH MED,DEPT HUMAN GENET,PHILADELPHIA,PA 19104
[3] WISTAR INST,PHILADELPHIA,PA 19104
[4] FRED HUTCHINSON CANC RES CTR,DEPT GENET,SEATTLE,WA 98104
来源：
SCIENCE | 1992年 / 255卷 / 5052期
关键词：
D O I：
10.1126/science.1372755
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Id is a helix-loop-helix (HLH) protein that represses activity of several basic helix-loop-helix (bHLH) proteins involved in cell type-specific transcription and cell lineage commitment. The myeloid precursor cell line 32DC13(G) expressed Id messenger RNA, which was transiently decreased when cells were induced to terminally differentiate with granulocyte-colony-stimulating factor. Concomitant with the decrease of Id messenger RNA was the appearance in nuclear extracts of DNA binding proteins that recognized a canonical E-box motif, a DNA binding site for some bHLH proteins. Constitutive expression of an Id complementary DNA in 32DC13(G) cells blocked their ability to differentiate and to induce E-box-binding activity. These results suggest that Id and, hence, bHLH proteins function in the process of myeloid differentiation.
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收藏
页码：1700 / 1702
页数：3
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